hahn lab

biosensor development and design

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Daglilyan, O., Krokhotin, A., Ozkan-Dagliyan, I., Deiters, A., Der, C.J., Hahn, K.M. and Dokholyan, N.V. Computational design of chemogenetic and optogenetic split proteins. Nature Communications, 9: 1-8, 2018. Doi: 10.1038/s41467-018-06531-4. online article | Free PMC article

*Hodgson, L., *Spiering, D., Sabouri-Ghomi, M., DerMardirossian, C., *Danuser, G., and *Hahn, K.M. FRET binding antenna reports spatioteGDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10): 802-9, 2016. PMC5030135. Online article | Free PMC article

MacNevin, C.J., Toutchkine, A., Marston, D.J., Hsu, C.W., Tsygankov, D., Li, L., Liu, B., Qi, T., Nguyen, D.V. and Hahn, K.M. Ratiometric imaging using a single dye enables simultaneous visualization of Rac1 and Cdc42 activation. J. Am. Chem. Soc., 138(8): 2571-2575, 2016. PMC4825053. Online article | Free PMC article

Zawistowski, J.S., Sabouri-Ghomi, M., Danuser, G., *Hahn, K.M., and *Hodgson, L. A. RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells. PLoS One 8(11): e79877, 2013. PMC3818223. Online article | Free PMC article

Kummer, L., Hsu, C.W., Dagliyan, O., MacNevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N.V., Hahn, K.M., Plückthun, A.  Knowledge-Based Design of a Biosensor to Quantify Localized ERK Activation in Living Cells.  Chem. Biol. 20(6): 847-56, 2013. PMC415710. Online article | Free PMC article

Jha, R.K., Wu, Y.I., Zawistowski, J., MacNevin, C., *Hahn, K.M. and *Kuhlman B. Redesign of the PAK1 auto-inhibitory domain for enhanced stability and affinity in biosensor applications. J. Mol. Biol., 413(2): 513-522, 2011. PMC3202338. Online article | Free PMC article

Gulyani, A., Vitriol, E., Allen, R., Wu, J., Gremyachinskiy, D., Lewis, S., Dewar, B., Graves, L.M., Kay, B.K., Kuhlman, B., Elston T., and Hahn, K.M.  A biosensor generated via high-throughput screening quantifies cell edge Src dynamics. Nature Chem. Biol., 7: 437-444, 2011.  PMC3135387. Online article. | Free PMC article

Shadpour, H., Zawistowski, J.S., Herman, A., Hahn, K.M., and Allbritton, N.L.  Patterning pallet arrays for cell selection based on high-resolution measurements of fluorescent biosensors. Analytica Chimica Acta, 696: 101-107, 2011. PMC3104244. Online article | Free PMC article

Machacek, M., Hodson, L., Welch, C., Elliot, H., Pertz, O., Nalbant, P., Abell, A., Johnson, G., *Hahn, K.M. and *Danuser, G. Coordination of Rho GTPase activities during cell protrusion. Nature, 461:99-103, 2009. PMC2885353. Online article | Free PMC article

Garrett, S.C., Hodgson, L., Rybin, A., Toutchkine, A., Hahn, K.M., Lawrence, D.S., and Bresnick, A.R. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor screening and Cellular Activation Dynamics. Biochemistry, 47:986-996, 2008. PMC3227476. Online article | Free PMC article

Hodgson, L., Pertz, O., and Hahn, K.M. Design and Optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol, 85:63-81, 2008. [Link downloads PDF] Online article

Cai, X., Lietha, D. Ceccarelli, D.F., Karginov, A.V., Rajfur, Z., Jacobson, K., Hahn, K.M., Eck, M.J., and Schaller, M.D. Spatial and temporal regulation of focal adhesion kinase activity in living cells. Mol. Cell Biol. 28:201-14, 2008. PMC2223290. Online article | Free PMC article

Hodgson, L., Chan, E.W.L., Hahn, K.M., and Yousaf, M.N. Combining Surface Chemistry with a FRET-Based Biosensor to Study the Dynamics of RhoA GTPase Activation in Cells on Patterned Substrates. J. Am. Chem. Soc. 129:9264 - 9265, 2007. Online article | PDF

Hodgson, L. Nalbant, P. Shen, F., and Hahn, K. Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation. Methods Enzymol., 406:140-156, 2006. Online article | PDF`

Pertz, O., L. Hodgson, R. R. Klemke, and K.M. Hahn. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature,  440(7087):1069-1072, 2006. Online article | PDF

Nalbant, P., L. Hodgson, V. Kraynov, A. Toutchkine, K.M. Hahn. Activation of Endogenous Cdc42 Visualized in Living Cells. Science, 305:1615-1619, 2004. Online article | PDF

Tan, X., Nalbant, P., Toutchkine, A., Hu, D., Vorpagel, E.R., Hahn, K.M. and Lu, H.P. Single molecule study of protein-protein interaction dynamics in a cell signaling system. J. Phys. Chem. B, 108: 737-744, 2004. [Link downloads PDF]

Weiss, T. S., C. E. Chamberlain, T. Takeda, P. Lin, K. M Hahn and M. G. Farquhar. Live-Cell fluorescence resonance energy transfer (FRET) shows Interaction between Gai3 on golgi membranes and the cytosolic pool of calnuc. Proc. Natl. Acad. Sci., USA. 98:14961-14966, 2001. PMC64966. Online article | Free PMC article

Kraynov, V. S., Chamberlain, C. E., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and K. M. Hahn. Localized Rac activation dynamics visualized in living cells. Science, 290:333-337, 2000. Online article | PDF

Chamberlain, C.E., Kraynov, V. and Hahn, K.M. Imaging spatiotemporal dynamics of Rac activation in vivo with FLAIR. Methods Enzymol., 325: 389-400, 2000. Online article | PDF

Bark, S. J., and K.M. Hahn. Fluorescent indicators of peptide cleavage inside living cells. Methods, 20: 429-435, 2000. Online article | PDF

Hahn, K.M., R. DeBiasio and D.L. Taylor.  Patterns of elevated free calcium and calmodulin activation in living cells. Nature, 359:736-738, 1992. Online article | PDF

Hahn, K.M., A.S. Waggoner and D.L. Taylor.  A calcium-sensitive fluorescent analog of calmodulin based on a novel calmodulin- binding fluorophore.  J. Biol. Chem., 265: 20335-20345, 1990. Online article | PDF

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