hahn lab

biosensor with binding antenna

Design and validation of GDI.Cdc42 FLARE, a genetically encoded biosensor that reports localization of Cdc42-GDI complexes. The FRET of the “binding antenna” on Cdc42 is altered when Cdc42 binds endogenous GDI. 

*Hodgson, L., *Spiering, D., Sabouri-Ghomi, M., DerMardirossian, C., *Danuser, G., and *Hahn, K.M. FRET binding antenna reports spatioteGDI-Cdc42 GTPase interactions. Nature Chem. Biol., 12(10): 802-9, 2016. PMC5030135. Online article | Free PMC article

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